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• Ready-to-use—supplied in a loading buffer for direct loading on gels; no need to boil. Each lot of the PageRuler Prestained Protein Ladder is calivrated against a precisely sized, unstained PageRuler Protein Ladder and calculated apparent molecular weights are reported in the picture. A blue chromophore is bound to all proteins, except proteins of two reference bands of 70 kDa and 25 kDa that are colored with an orange dye and one green reference band of 10 kDa. Approved for shipment on Wet or Dry Ice. PageRuler Prestained Protein Ladder is a mixture of 10 recombinant, highly purified colored proteins with the apparent molecular weights from 10kDa to 170kDa. Jordan Journal of Biological Sciences, 2020, 13. For 100 mini-gel applications 5ul per well or 50 large gel applications 10ul per well).
The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use. Storage: Upon receipt store at -20°C. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Characterization of Egyptian durum Wheat Genotypes using Biochemical and Molecular Markers. 5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol. Features of PageRuler Prestained Protein Ladder: • Size range—10 proteins spanning 10 to 180 kDa. 5ul of protein ladder samples was loaded into each lane. Please try the standard protocols listed below and let us know how you get on.
Evaluated by SDS-PAGE and Western blotting. Organization: Institute for Biomedic Research of A Coruna (INIBIC). Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. ▋JIANG, Yan-Yi, et al. • Quality tested—each lot evaluated by SDS-PAGE and Western blotting. Blu10 Plus (BLUltra) Prestained Protein Ladder(6. Opakowanie||2x250 µl|. Global Journal of Biotechnology and Biomaterial Science, 2020, 6. 3 colors: Blue, Orange, Green. Purification and some properties of fungal Xylanase. Our Abpromise guarantee covers the use of ab286875 in the following tested applications. Stable for up to 24 months at -20°C. Arab Journal of Basic and Applied Sciences, 2022, 29.
PURIFICATION, MOLECULAR AND BIOCHEMICAL CHARACTERIZATION AND BIOLOGICAL APPLICATIONS OF HEMAGGLUTINATING LECTIN WITH ANTICANCER ACTIVITIES FROM PLEUROTUS OSTREATUS. A facile method for high level dual expression of recombinant and congener protein in a single expression system. Lane 8: PMU12-0500 (Bio-Helix) 5ul. Packaging: Kit includes dye-stained proteins in 62, 5 mM Tris-H₃PO₄ (pH 7, 5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃ and 33% glycerol. ▋ABDUL AZIZ, Marwa, et al. ► Better performance for higher molecular weight proteins. Protein ladders (pre-stained), PageRuler™ plusSupplier: Thermo Scientific. Effect of zinc oxide nanoparticles on the growth, genomic DNA, production and the quality of common dry bean (Phaseolus vulgaris). Bulletin of the National Research Centre, 2020, 44. Lane 9: Thermo Scientific 26616 PageRuler Prestained Protein Ladder / 10μl. Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm). TP63, SOX2, and KLF5 establish a core regulatory circuitry that controls epigenetic and transcription patterns in esophageal squamous cell carcinoma cell lines. Two vials of 250 µL each.
Size Range: 10 to 180 kDa. • In low-percentage gels (< 10%), the low-molecular w eight proteins in the ladder may migrate with the dye front. Not sure which protein marker to choose? Design, production and purification of a novel recombinant gonadotropin-releasing hormone associated peptide as a spawning inducing agent for fish. Quantity: 2 x 250 μL. Acta Ecologica Sinica, 2020, 40. 5mM Tris-H3PO4 (pH 7. Sprawdź także MWP03............................................................ Wcześniejszy numer katalogowy: SM0671. Load the following volumes of the ladder on an SDS-polyacrylamide gel: – 5 μl per well for mini gel, – 10 μl per well for large gel. Guide for Molecular Weight Estimation (kDa).
Additional data should be considered for a more accurate adjustment. Load the Ladder on an SDS-PAA gel and run. The ladder contains one orange reference band at 70kDa and one green band at 10kDa. Application||Abreviews||Notes|.
Tested applicationsSuitable for: SDS-PAGE, WB more details. 007% 2-Mercaptoethanol, 21. Genetic relationship study of some Vicia species by FISH and total seed storage protein patterns. The influence of He-Ne laser on agro-morphological criteria, ISSR marker and SDS-PAGE of Moringa oleifera.